Farah Deeba, PhD student at University of Punjab (Pakistan)
David W. Wood, faculty mentor
Pathogenic fungi (Alternaria alternata, Penicillium digitatum, Neurospora sp., Aspergillus fumigatus) cause severe damage to fruits and vegetables and are responsible for considerable annual low yields. This research seeks to test safer and environmentally more sound biological control measure to control these pathogenic fungi as compared to chemically harmful fungicides.
Research was conducted to develop a low cost and time effective bacterial chitinase production process. The best bacterial chitinase producers were screened, identified and the chitinases were partially purified (ammonium sulfate precipitation) and then purified by ionic exchange chromatography. The minimum effective inhibition concentration of each chitinase was estimated against four different fungi.
To provide an eco-friendly, cost effective natural biological approach for the control of fungi to prevent fruit and vegetable damage.