The role of CD200R signaling in small intestinal γδ+ intraepithelial lymphocytes

Jianyu Yu (China)
Xue-Feng Bai, faculty mentor

Background

• Hometown: Guangdong, China
• Degrees received: Medical Degree in gastroenterology, Southern Medical University, China

What is the issue or problem addresses in your research?

CD200R, the cognate ligand for CD200, is an IgSF protein expressed on several types of immune cells in mouse and human, including macrophages, neutrophils and mast cells. While a few reports revealed that CD200R signaling plays a protective role in inflammatory bowel disease (IBD), very few evident uncover the expression of CD200R in the the gastric tract or the relationship between CD200R signaling and the gut immune system, which plays a key role in IBD.

What methodology did you use in your research?

• Animals: female C57BL/6J or CD200R-KO mice aged
• Intestinal lymphocytes were isolated by Digest buffer and Density gradient centrifugation
• Lymphocytes population of spleen, Peyer's patches, mesenteric lymph nodes (MLN), small intestine and colon was detected by Flow cytometry
• CD200R expression was detected by Flow cytometry
• Cell proliferation was detected by in-vivo injection of BrdU and Flow cytometry

What are the purpose/rationale and implications of your research?

Our main goal is to study: 1) on which type of intestinal lymphocytes is CD200R expressed mainly; 2) Why CD200R expression is up regulated; 3) How CD200R signaling impact intestinal lymphocytes population and their functions. Our results suggest that CD200R is expressed variously in different types of intestinal immune cells and plays an important role in intestinal lymphocyte population and cell proliferation.