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Establish expression and function CFTR in immune cells

Research Scholar

Zayed Attia, assistant lecturer of internal and infectious animal diseases, Sadat City University (Egypt)
Prosper Boyaka, Faculty Mentor

Background

  • Hometown: Sadat Cityt, Egypt
  • Degrees received: Master of Science in internal and infectious animal diseases, Sadat City University, Egypt.

What is the issue or problem addresses in your research?

Cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel that plays a key role in fluid homeostasis in the lung due to its expression in airway epithelial cells. Mutations in the cftr gene leading to absence of functional CFTR result in Cystic Fibrosis (CF), a progressive disease that affect multiple organs including the lungs and the gastro-intestinal tract. Most of the CF patients exhibit chronic lung infection and inflammation, which eventually results in respiratory failure. CFTR biology has extensively been studied in epithelial cells but its role in immune cells remains elusive. Immune cells play a key role in controlling infections.

What methodology did you use in your research?

  • Animals: female C57BL/6J or CFTR-KO mice aged
  • Immunophenotyping of cell population spleen and mesenteric lymph nodes (MLN) cells were analyzed for immunophenotyping by Flow cytometry
  • Cells were cultured for with or without stimulation for immunoglobulins class switching
  • After culture, cells were stained and analyzed by Flow cytometry or ELISA for total Ab secreted

What are the purpose/rationale and implications of your research?

The main goal of this study is to 1) determine whether immune cells express CFTR; 2) investigate whether absence of functional CFTR alter immune cell function; and 3) defective immune response to infection as well as vaccines could explain why CF patients are more susceptible to infection and are not protected by vaccines.