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N-Butanol production from engineered Clostridium cellulovorans

Research Scholar

Wenjie Hou, lecturer, Northwest A & F University (China)
Shang-Tian Yang, faculty mentor


  • Hometown: Shaanxi, China
  • Degrees received: PhD in microbiology, Northwest A & F University, China.

What is the issue or problem addresses in your research?

Finding a potential biomass for n-butanol production, especially pretreatment and enzymatic hydrolysis materials are essential steps to obtain fermentable sugars because of the recalcitrant nature of lignocellulose, which usually results in a high operational cost. A consolidated bioprocess (CBP) was developed using a metabolically engineered Clostridium cellulovorans adhE2. Various saccharides served as substrates for n-butanol production by Clostridium cellulovorans adhE2.

What methodology did you use in your research?

Different carbon sources include glucose, xylose, cellulose, xylan and xylooligosaccharide, were investigated. The results showed that n-butanol can be produced from all substrates studied, including cellulose and xylan. And faster growth appeared in media containing glucose and xylose.

What are the purpose/rationale and implications of your research?

This study demonstrated that the fermentation process with the engineered Clostridium cellulovorans enabled a potential n-butanol production directly from lignocellulosic biomass.