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Drum Priming of Bell Pepper Seeds(Capsicum annuum L.) Using Bioregulator

Research Scholar

Clissia Barboza de Silva, Department of Horticulture and Crop Science (Brazil)
Pablo Jourdan, Faculty Mentor
Mark Alan Bennett, Co-Researcher


Clissia Barboza de Silva received her undergraduate degree in Agronomy from the Federal University of Alagoas, Brazil in 2009, specializing in Seed Technology. In 2011 she obtained the Master's degree in Crop Science with a focus in Seed Technology from the Sao Paulo State University Brazil. Currently, Silva is a PhD student in Plant Science with emphasis in Seed Technology at the University of Sao Paulo, and has a scholarship from the Sao Paulo Research Foundation to pursue a research project at The Ohio State University as a visiting scholar under the supervision of Dr. Pablo Jourdan.

What is the issue or problem addressed in your research?

Bell pepper seeds are characterized by slow germination and low uniformity. Drum priming is a special imbibition treatment for seeds that has been shown to enhance germination and uniformity of various seed crops by influencing the early stages of germination. Combining drum priming methods with bioregulators that have been shown to be active in seeds may lead to further enhancement of germination and stand establishment by stimulating both physiological parameters such as faster development of seedlings and perhaps provide resistance to environmental stresses such as high temperatures, drought and salinity.

What methodology did you use in your research?

Drum priming has been used to control the imbibition process in seeds; in this research I have been using a novel bioregulator, 24-epibrassinolide, in the priming process. This is a new type of plant growth regulator used in agriculture that appears to cause physiological and biochemical changes in seeds such as increase on seedling growth and activity of enzymes responsible for the removal toxic substances from the seeds. These enzymes are being evaluated in this research after priming with 24-epibrassinolide. In addition, the software “Seed Vigor Imaging System-SVIS®” developed by The Ohio State University, is being used to obtain information about physiological parameters related to the seedling growth potential.

What are the purpose/rationale and implications of your research?

Improvement in crop production efficiency is essential to insure a sustainable level of food crops for an expanding population. Development of new tools, such as priming with growth regulators, that contribute to the production system of vegetable seeds has been essential in the global seed industry, assisting producers and contributing to the release of high quality seeds. If priming with 24-epibrassinolide enhances bell pepper seed germination and seedling establishment, there will be the possibility of commercial distribution of higher quality seeds, ameliorating problems faced by producers such as the constant necessity of re-sowing, bringing thereby advantages for the production economy.